assembly software package Search Results


90
DNASTAR assembly software package
Assembly Software Package, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assembly software package - by Bioz Stars, 2026-04
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Celera software packages for whole genome assembly
Software Packages For Whole Genome Assembly, supplied by Celera, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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software packages for whole genome assembly - by Bioz Stars, 2026-04
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Bioedit Company assembly software package
Assembly Software Package, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assembly software package - by Bioz Stars, 2026-04
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DNASTAR seq-man sequence assembly software package, version 5.07
Seq Man Sequence Assembly Software Package, Version 5.07, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novogene short-read assembly program for avocado in the trinity software package (v. r2014043p1)
Short Read Assembly Program For Avocado In The Trinity Software Package (V. R2014043p1), supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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short-read assembly program for avocado in the trinity software package (v. r2014043p1) - by Bioz Stars, 2026-04
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DNASTAR assembler 7 software package
HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp (HBV sequence 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. <t>Ligated</t> <t>DNAs</t> introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using <t>SeqMan</t> assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.
Assembler 7 Software Package, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assembler 7 software package - by Bioz Stars, 2026-04
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10X Genomics software package for de novo assembly from chromium linked-reads
HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp (HBV sequence 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. <t>Ligated</t> <t>DNAs</t> introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using <t>SeqMan</t> assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.
Software Package For De Novo Assembly From Chromium Linked Reads, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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software package for de novo assembly from chromium linked-reads - by Bioz Stars, 2026-04
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Biotechnology Information bwa software package mm9 national center for biotechnology information assembly of the mus musculus genome
HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp (HBV sequence 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. <t>Ligated</t> <t>DNAs</t> introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using <t>SeqMan</t> assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.
Bwa Software Package Mm9 National Center For Biotechnology Information Assembly Of The Mus Musculus Genome, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bwa software package mm9 national center for biotechnology information assembly of the mus musculus genome/product/Biotechnology Information
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bwa software package mm9 national center for biotechnology information assembly of the mus musculus genome - by Bioz Stars, 2026-04
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DNASTAR pro assembler software package dnastar® lasergene® version 7.2
HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp (HBV sequence 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. <t>Ligated</t> <t>DNAs</t> introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using <t>SeqMan</t> assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.
Pro Assembler Software Package Dnastar® Lasergene® Version 7.2, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pro assembler software package dnastar® lasergene® version 7.2 - by Bioz Stars, 2026-04
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HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp (HBV sequence 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. Ligated DNAs introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using SeqMan assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.

Journal: Antiviral research

Article Title: Distinct dual antiviral mechanism that enhances hepatitis B virus mutagenesis and reduces viral DNA synthesis

doi: 10.1016/j.antiviral.2019.104540

Figure Lengend Snippet: HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp (HBV sequence 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. Ligated DNAs introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using SeqMan assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.

Article Snippet: Recombinant plasmid DNAs were purified and sequenced, with alignments being done by using the SeqMan assembler of the Lasergene 7 software package (DNAStar; Madison, WI).

Techniques: Cell Culture, Purification, Sequencing, Amplification, Gel Purification, Plasmid Preparation, Transformation Assay, DNA Sequencing, Software, Mutagenesis